Fig. 3

trans-Resveratrol induces ROS production and lipid peroxidation. a MCF-7 cells were incubated with 50 μM DHCF-DA at 37°C for 30 min and then treated with 50 μg/ml trans-resveratrol up to 6 h. At the indicated time points, cells were washed with PBS and ROS production was analysed by FACScalibur instrument. Data are expressed as mean ± SD (n = 3) **P < 0.001. b MCF-7 cells were treated for 24 h with 50 μg/ml trans-resveratrol. Lipid peroxidation was evaluated by measuring the levels of lipid peroxidation by-products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) using a colorimetric method. Data are expressed as means ± SD. (n = 5). **P < 0.001 versus untreated cells