Fig. 6

JNK and p38^MAPK are differently responsible for trans-resveratrol-induced apoptosis. MCF-7 cells were incubated with 10 μM SP600125 or SB203580, the specific inhibitors of JNK and p38^MAPK, respectively, for 1 h before and throughout the treatment with 50 μg/ml trans-resveratrol. a After 24 h treatment, cells were stained with propidium iodide for cytofluorimetric analyses. Percentages of apoptotic cells are expressed as means ± S.D. (n = 5). **P < 0.001. b. After 18 h 20 μg of cytosolic extracts were loaded onto each lane for detection of cytochrome c. β-actin was used as loading control. Immunoblots are from one experiment representative of three that gave similar results. Densitometric analyses of each lane were calculated using Quantity One Software and data are expressed as arbitrary units. c Alternatively, cells were grown on chamber-slices and treated for 18 h. After washing with PBS and fixing with 0.4% paraformaldehyde, cells were stained with Hoechst 33342 and visualized by fluorescence microscopy. White arrows indicate condensed chromatin or apoptotic bodies after trans-resveratrol treatment