Increased inflammatory response in aged mice is associated with age-related zinc deficiency and zinc transporter dysregulation

doi:10.1016/j.jnutbio.2012.07.005

PMC full text:

J Nutr Biochem. Author manuscript; available in PMC 2014 Jan 1.

Published in final edited form as:

J Nutr Biochem. 2013 Jan; 24(1): 353–359.

Published online 2012 Sep 13. doi: 10.1016/j.jnutbio.2012.07.005

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Fig 1

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Proinflammatory response was associated with reduced intracellular zinc and was enhanced by zinc deficiency. THP-1 cells (n=3 per treatment) were cultured in ZA or ZD media for 10 to 13 days. Total zinc (A) and intracellular zinc (B) were determined by ICP-OES and FluoZin-3 flow cytometry, respectively. Intracellular zinc and proinflammatory response were measured in ZA or ZD THP-1 cells that were left untreated (UT), or stimulated with 10 or 100 ng/ml LPS for 6 h. Expressions of TNFα (C) and IL1β mRNA (D) were determined by real-time PCR. Data represent mean±S.E.M. MFI, mean fluorescence intensity. *P<.05 vs. ZA. Results are representative of three independent experiments.