| PMC full text: | J Nutr Biochem. Author manuscript; available in PMC 2014 Jan 1. Published in final edited form as: J Nutr Biochem. 2013 Jan; 24(1): 353–359. Published online 2012 Sep 13. doi: 10.1016/j.jnutbio.2012.07.005 |
Fig 2
Age-related reduction in immune cell intracellular zinc levels was associated with enhanced proinflammatory responses. Plasma zinc (A) and intracellular zinc (B) in various immune cells including thymocytes, splenocytes, BM cells and BMDCs were determined in groups of young (2 months) and aged (26 months) mice (n=5 per age group) by ICP-OES and FluoZin-3 flow cytometry, respectively. (C) IL1β mRNA expression in the spleens of mice at various ages (n=10 per age group) were determined by real-time PCR. (D) Splenocytes from young (2 months) and aged (26 months) mice (n=4 per age group) were left untreated, or stimulated with 0.1 or 1 μg/ml LPS for 6 h. IL1β mRNA expression was determined by real-time PCR. Data represent mean normalized fold-change±S.E.M. vs. young mice (2 months; A, B and D) or 3-month-old mice (C). *P<.05 vs. young mice. NS, not significant.